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    Laboratory Methods in Enzymology: Protein Part C

    Laboratory Methods in Enzymology: Protein Part C

    - Volume 541

    Forfatter: info mangler
    Bog
    • Format
    • Bog, hardback
    • Engelsk

    Beskrivelse

    In this volume we have brought together a number of core protocols concentrating on Protein, carefully written and edited by experts.

    Læs hele beskrivelsen
    Detaljer
    • SprogEngelsk
    • Sidetal296
    • Udgivelsesdato09-06-2014
    • ISBN139780124201194
    • Forlag Academic Press Inc
    • FormatHardback
    Størrelse og vægt
  • Vægt590 g
    • coffee cup img
    10 cm
    book img
    15,1 cm
    22,9 cm

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    Eukaryotic cells Membrane proteins Affinity chromatography Protein-protein interactions Schizosaccharomyces pombe Antigen-antibody reactions Detergents Ion exchange chromatography Prokaryotic Cells Solubilization Binding Dialysis Eukaryotic expression systems Protein purification Poly(acrylamide) Hybridoma Size-exclusion chromatography SDS-PAGE UV/Vis Spectroscopy Protein/nucleic acid interactions Glycerol In vitro assays Protein transfer Immunoblotting Western blotting EGF receptor Gel filtration chromatography PH measurement Resin Salting-out Cell Free Expression Systems Adsorbent regeneration and sanitization Ammonium persulfate (APS) Ascitic Fluid Antibody and resin preparation Binding partner detection Ammonium sulfate Carboxymethyl (CM) Bromophenol blue Cell lysate Calcium ion analysis Chaotropes Deoxycholate-trichloroacetic acid precipitation Differential scanning fluorimetry (DSF) Cell lysis Coomassie blue staining Dithiothreitol (DTT) E. coli cell growth Diethylaminoethyl (DEAE) Coimmunoprecipitation Column volume Column equilibration and loading G-250 FPLC system Coupling antibody to resin Gel filtration column Gel shift assays Cyanogen bromide (CNBr)-activated Sepharose Hofmeister Series Horseradish peroxidase (HRP) Hydroxyapatite Chromatography IPTG hydrophobic interaction chromatography (HIC) Kimwipes or paper towels Immunoprecipitations Electrophoretic mobility shift assay (EMSA) Ion exchange chromatography (IEX) Lyotropes Miniprep DNA Nitrocellulose membrane Normalization of cell lysates Nytranâ„¢ SuPerCharge (SPC) membranes One-dimensional SDS-Polyacrylamide Gel Electrophoresis (1D SDS-PAGE) Polyvinylidene fluoride (PVDF) membrane Protein A-sepharose Optimal solubilization Quaternary aminoethyl (QAE) R-250 RNA and DNA binding Polyacrylamide gel electrophoresis Polyacrylamide gels (PAGE) Protein filter binding Sodium dodecyl sulfate polyacrylamide gel electrophoresis Linear and circular dichroism Surface neutralization solutions (SNS) SEC profile mAb fraction Silver staining Hydrophobic proteins SDS-PAGE mini-gel Void volume Trichloroacetic acid (TCA) Whole cell lysates Ni-NTA Superflow Polyacrylamide gel preparation Macromolecular bands Nucleic acid-protein interaction SDS-PAGE gel SDS-polyacrylamide gel Poly(A)-binding protein (PABP) Purification screening protocol Phosphoryl groups T7 expression vectors T7 RNA Polymerase Tetramethylethylenediamine (TEMED) XL1-Blue cells Troubleshooting protein expression Protein solubility Quaternary anion (Q) Small ubiquitin-like modifier (SUMO) Sodium dodecyl sulfate (SDS) Whatman 1 filter paper Stacking gel Tris base Sulfopropyl (SP) Technology in HIC adsorbents Whatman GF/C filters

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