Favoritter
    Log ind Opret dig
    Studiebog
    Laboratory Methods in Enzymology: Protein Part B

    Laboratory Methods in Enzymology: Protein Part B

    - Volume 539

    Forfatter: info mangler
    Bog
    • Format
    • Bog, hardback
    • Engelsk

    Beskrivelse

    Laboratory Methods in Enzymology: Protein Part B brings together a number of core protocols concentrating on protein, carefully written and edited by experts.

    Læs hele beskrivelsen
    Detaljer
    • SprogEngelsk
    • Sidetal240
    • Udgivelsesdato29-04-2014
    • ISBN139780124201200
    • Forlag Academic Press Inc
    • FormatHardback
    Størrelse og vægt
  • Vægt650 g
    • coffee cup img
    10 cm
    book img
    15,2 cm
    22,9 cm

    Findes i disse kategorier...

    Se andre, der handler om...

    Plasmids Chromatography RNA-protein interactions Centrifugation French press Chromatin immunoprecipitation (ChIP) CDNA library preparation Cell-free protein synthesis Western blotting Immunoprecipitation 3-Aminotriazole (3-AT) concentration cDNA Library cDNA library preparation/reverse transcription Cell mixing Barcode sequences Autoactivation test and sequencing Back-transformation of yeast Co-immunoprecipitation (co-IP) assays Cell lysate preparation Electroporation of E. coli Cell lysis Cell-free translation Crosslinking and immunoprecipitation (CLIP) protocol DNA-binding domain (DB) fusion protein DNA-binding site Elimination of RNA-independent false positives Cloning and transformation methods Dithiothreitol (DTT) and phenylmethylsulfonyl fluoride (PMSF) Formaldehyde-fixed Caenorhabditis elegans Confirmation of positive interactors HIS3 and lacZ activation Hybrid RNA library Hydrolysis of NHS ester Keyword3-Aminotriazole (3-AT) resistance assay Immunoprecipitation and second RNase T1 digestion Key word3-AT concentration Key wordAmount of BS3 Key wordActivation domain (AD) fusion protein Key wordAmino acids In vitro protein synthesis Key wordCross-linking protocol (CLIP) Mutant and control proteins Growth and harvest E. coli Optimization of coupled transcription and translation reaction Illumina Genome Analyzer PCR amplification of cDNA library pDBLeu-X β-Galactosidase activity Plasmid rescue Proteinase K treatment RNA immunoprecipitation (RIP) and cell-mixing protocols protein cross-linking Preparation of yeast cell-free extract Ribonucleoprotein particles (RNPs) Saccharomyces Cerevisiae cells Proteinase K digestion Key wordAnnealing protocol Radiolabeled translation products Solutions and buffers S30 preparation Self-activation of pDBLeu-X (and pPC86-Y) RNA-protein interaction Human Genome Project (HGP) Small nucleolar ribonucleoprotein particle (snoRNP) Small-scale yeast transformation Key word3′- and 5′-adapter ligation Transformation efficiency TCA Precipitation UV cross-linking Z-buffer Moisture condensation Northern blotting RNA plasmid Room temperature PCR primer preparation SDS-PAGE gel Phosphate-buffered Saline (PBS) Northern and Western blotting Posttranscriptional regulation (PTR) Sodium dodecyl sulfate (SDS) Lysis Buffer Renilla luciferase mRNA Yeast three-hybrid system Transcription factor (TF) Protein G magnetic beads Yeast two-hybrid screen UV crosslinking of 4-thiouridine-labeled cells Three-hybrid system

    Velkommen til Saxo – din danske boghandel

    Hos os kan du handle som gæst, Saxo-bruger eller Saxo-medlem – du bestemmer selv. Skulle du få brug for hjælp, sidder vores kundeservice-team klar ved både telefonerne og tasterne.

    Om medlemspriser hos Saxo

    For at købe bøger til medlemspris skal du være medlem af Saxo Premium, Saxo Shopping eller Saxo Ung. De første 7 dage er gratis for nye medlemmer. Medlemskabet fornyes automatisk og kan altid opsiges. Læs mere om fordelene ved vores forskellige medlemskaber her.

    Machine Name: SAXO082