Flow and Image Cytometry

Image analysis and flow cytometry are complementary techniques which provide new insights into essential aspects of cell biology. This book presents an up-to-date overview of current ideas and methods in these domains. It consists of three parts. Part I, 'Membrane Dynamics and Function', deals with adhesion molecules, membrane pump dynamics, membrane fusion and endocytosis, membrane potential, T-cell homing and cytoskeleton, etc. The second part focuses on problems of cell proliferation, chromosome analysis and sorting, apoptosis, imaging cytometry of fixed and living cells, image analysis for gene mapping from flow-sorted chromosomes, chromatin organization, and liposome-mediated delivery of DNA binding drugs. The third part covers data management systems, cell sorting techniques and microscopy.