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Section I: Introduction to BiofilmsChapter 1: Biofilm formation and pathogenesisChapter 2: Sample preparation, basic equipments and common laboratory assay protocols for Bacterial Communities and Biofilms Section II: Separation methods and Spectroscopic techniques in Biofilm researchChapter 3: Analysing microbial biofilm formation at a molecular level: Role of Fourier-Transform Infrared and Raman spectroscopyChapter 4: In vitro metabolite profiling of microbial biofilm: Role of Gas Chromatography and High Performance Liquid ChromatograpySection III: Electroanalytical techniques for evaluation of biofilm researchChapter 5: Microbial electrochemical cells and introduction to Electron transport in microbial biofilmChapter 6: Contribution of Cyclic voltammetry and electrochemical impedance spectroscopy in deciphering the electron transport system in biofilmSection IV: Calorimetric techniques in biofilm researchChapter 7: Measuring metabolism and bio-energetic profiles of biofilm: Isothermal calorimetry, Differential Scanning Calorimetry and future of Chip CalorimetrySection V: In Situ Surface Analysis and Imaging techniques in Biofilm researchChapter 8: Analysis of biofilm matrix by Multiplex Fluorescence In Situ Hybridization (M-FISH) and Confocal Laser Scanning Microscopy (CLSM) during nosocomial infectionsChapter 9: Imaging Bacteria and biofilm by Field Emission Scanning Electron Microscopy (FESEM) Chapter 10: Probing the Surface-Attached in Vitro microbial Biofilms with Atomic Force (AFM) and Scanning probe microscopy (SPM).Chapter 11: Nuclear Magnetic Resonance (NMR) and Surface Plasmon Resonance (SPR) imaging as an advanced tool for examining biofilm matrix (Structure, Composition, Dynamics)Section VI: Molecular Biology and recombinant DNA techniques in Biofilm researchChapter 12: Genetic Basis of Biofilm formation and spread of nosocomial infectionsChapter 13: Monitoring cell distribution and apoptosis in sessile forms of microbial biofilm: Flow cytometry -fluorescence activated cell sorting (FCM-FACS)Chapter 14: Monitoring gene expression in sessile forms of microbial biofilm: Polymerase chain reaction (PCR) and Real Time Polymerase chain reaction (RT-PCR)
