Advanced Methods in Molecular Biology and Biotechnology

- A Practical Lab Manual

  • Format
  • Bog, paperback
  • Engelsk

Beskrivelse

Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual is a concise reference on common protocols and techniques for advanced molecular biology and biotechnology experimentation. Each chapter focuses on a different method, providing an overview before delving deeper into the procedure in a step-by-step approach. Techniques covered include genomic DNA extraction using cetyl trimethylammonium bromide (CTAB) and chloroform extraction, chromatographic techniques, ELISA, hybridization, gel electrophoresis, dot blot analysis and methods for studying polymerase chain reactions. Laboratory protocols and standard operating procedures for key equipment are also discussed, providing an instructive overview for lab work. This practical guide focuses on the latest advances and innovations in methods for molecular biology and biotechnology investigation, helping researchers and practitioners enhance and advance their own methodologies and take their work to the next level.

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Detaljer
  • SprogEngelsk
  • Sidetal218
  • Udgivelsesdato04-11-2020
  • ISBN139780128244494
  • Forlag Academic Press Inc
  • FormatPaperback
Størrelse og vægt
  • Vægt460 g
  • coffee cup img
    10 cm
    book img
    19,1 cm
    23,5 cm

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    Separation DNA Transformation Laboratory Electrophoresis Equipment Molecular biology Nucleic acids Replication Thin layer chromatography Hybridization Proteins Affinity chromatography Gene amplification Adsorption Chromatography Bacterial transformation Signal detection Recombinant DNA Capture RNA Polymerase chain reaction DNA fingerprinting Electroporation Gel electrophoresis Denaturation Immobilization Protein page Vector Cation exchange DNA-POLYMERASE CDNA MRNA Analytes PCR Centrifuge Molecular sieve Operations Markers Southern blotting Annealing Hazards Blotting Sedimentation Amplification PVP Gene Cloning Procedures Protein extraction Western blotting Immunoassays Polypeptide chain Partition Competency Growth curve Enzyme linked immunosorbent assays Gel filtration chromatography Polymorphism Molecular weight Autoclave Agarose Immunodetection 2DE Analyte Absorbance Anion Exchange Amplicon Blocking Buffer Bacterial culture carrier Cuvette Death phase Binding buffer Eastern blotting Dansyl Chloride Density gradient Gene isolation Continuous-bed Fume cupboard Differential centrifugation Direct ELISA Indirect ELISA Edman Degradation Enzyme reaction lag phase Ionic resins Exponential phase Membrane strip Lysine residue Nitrocellulose membrane Mobile Phase Paper Chromatography Gel Permeation Sample spotting Isopycnic centrifugation Relative to front Primary Antibody Primer designing Sandwich ELISA Intersimple sequence repeats Sodium acetate SDS 29 sample buffer Lysine side chain Northern blotting Log phase Removal of interfering substances Secondary antibody Stationary Phase Retention factor Size exclusion Spectrophotometer TCA/acetone precipitation Zonal density gradient

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