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Bog, paperback Low-Cost Methods for Molecular Characterization of Mutant Plants af Bradley J. Till

Low-Cost Methods for Molecular Characterization of Mutant Plants

- Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols

(Bog, paperback)

6 black & white illustrations, 3 colour illustrations, biography

6 black & white illustrations, 3 colour illustrations, biography

Produktdetaljer:

Sprog:
Engelsk
ISBN-13:
9783319386676
Sideantal:
45
Udgivet:
04-11-2016
Udgave:
Softcover reprint of the original 1st ed. 2015
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Forlagets beskrivelse
6 black & white illustrations, 3 colour illustrations, biography
Bibliotekernes beskrivelse
This book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extraction of single-strand-specific nucleases for single nucleotide polymorphism; and small insertion/deletion discovery using standard agarose gel electrophoresis. The methods described can be applied in any laboratory equipped for basic molecular biology and do away with the need for expensive freezers and toxic organic compounds. With the appropriate validation of sample quality and longevity, they can provide sufficient DNA for a variety of molecular applications, such as marker studies and TILLING, at approximately one tenth of the cost per sample when compared to commercial kits.

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