Development and Validation of Indicators for the Production and Quality of Seed Cultures

- Gehder, M: Development and Validation of Indicators for the

af


Development and Validation of Indicators for the Production and Quality of Seed Cultures
Du sparer Spar kr. 55,00 med Shopping-fordele
  • Leveringstid 2-3 uger
  • Forventet levering 12-11-2021
For at købe bogen til fordelspris skal du have et medlemskab med Shopping-fordele.
Du kan prøve medlemskabet gratis i 30 dage. Medlemskabet fornyes automatisk og kan altid opsiges.
Format:
Bog, paperback
Udgivelsesdato:
31-08-2011
Sprog:
Engelsk
Sidetal:
182
  • Beskrivelse
  • Yderligere info
  • Anmeldelser

Cultivations of filamentous fungi are of large industrial and scientific interest. However, the utmost starting point of the majority of those cultivations, the inoculum consisting of fungal spores, has previously been neglected. This work hence introduces a total of nine spore quality indicators and subsequently correlates the sporulation environome to the properties of the generated spores and their performance in submerged cultivation. The model organism is Aspergillus ochraceus while the model process is the hydroxylation of a steroid. A positive correlation between the spore yield and the substrate¿s carbon content on solid medium was found with oat bran agar giving best yields of 26 107 spores/mL at carbon concentrations of 15.4 g/L in the solid medium. The assessment of the conidial metabolome revealed the presence of 126 metabolites. Particularly polyols, such as glycerol, erythritol and ribitol, are found at high concentrations of up to 55 ng/105 spores. It was further shown, that several of these metabolites are degraded within the spores during storage for 7 d in saline solution. Their concentrations also strongly correlate with the storage resistance of the conidia as well as with their performance in subsequent submerged cultivations. Highest reactant turnover of approx. 80 % was determined after 72 h of cultivation with spores generated on wheat bran agar. The culture performance is further a function of the portion of protein bound NAD(P)H, which was determined and spatially visualized by means of two-photon fluorescence lifetime imaging.

Vis mereVis mindre

Udgivelsesdato:
31-08-2011
ISBN13:
9783869558479
Vægt:
244 g
Dybde:
10 mm
Bredde:
148 mm
Højde:
210 mm
Format:
Paperback
Udgave:
Reihenbandnummer 59
Forfattere

Vis mereVis mindre

Vis mereVis mindre

Fandt du ikke hvad du søgte?

Hvis denne bog ikke er noget for dig, kan du benytte kategorierne nedenfor til at finde andre titler. Klik på en kategori for at se lignende bøger.

Velkommen til Saxo – din danske boghandel!

Hos os kan du handle som gæst, Saxo-bruger eller Premium-medlem – du bestemmer helt selv. Skulle du få brug for hjælp, sidder vores kundeservice-team klar ved både telefonerne og tasterne.

Om fordelspriser hos Saxo

For at købe bøger til fordelspris, skal du være medlem af Premium, Premium Shopping eller Premium Studie. De første 30 dage er gratis for nye medlemmer. Medlemskabet fornyes automatisk og kan altid opsiges. Læs mere om fordelene ved vores forskellige medlemskaber her.

Machine Name: SAXO082